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restriction enzyme afai  (TaKaRa)


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    Structured Review

    TaKaRa restriction enzyme afai
    Restriction Enzyme Afai, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/restriction+enzyme+afai/Afa+I/10__1007_slash_s10327___024___01202___3-44-34-37
    Average 93 stars, based on 131 article reviews
    restriction enzyme afai - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Amplification:

    Article Title: Pepper yellow leaf curl Aceh virus: a recombinant replacing the parental begomovirus is highly virulent and breaks Ty-1-mediated resistance in tomato
    Article Snippet: Begomovirus infections have threatened the global production of multiple crops, including Solanaceae species.. Recombination, a frequent phenomenon in begomoviruses, has profound effects on viral populations, allowing them to adapt to changing environments.. We previously isolated pepper yellow leaf curl Aceh virus (PepYLCAV) from pepper (Capsicum annuum), tomato (Solanum lycopersicum), and tobacco (Nicotiana tabacum) plants in Aceh Province, Indonesia, and determined that it is a recombinant of the widespread pepper yellow leaf curl Indonesia virus (PepYLCIV).

    Article Title: Aberrant methylation and histone deacetylation of cyclooxygenase 2 in gastric cancer.
    Article Snippet: .. After amplification, 20 l of product were digested with restriction enzyme AfaI (Takara) or TaiI (Fermentas). ..

    Article Title: Upregulation of BNIP3 by 5-aza-2'-deoxycytidine sensitizes pancreatic cancer cells to hypoxia-mediated cell death.
    Article Snippet: 1 First Department of Internal Medicine, Sapporo Medical University, South 1, West 16, Chuo-ku, Sapporo 060-8543, Japan 2 Department of Gastroenterology, Sapporo Kosei General Hospital, Sapporo, Japan 3 Department of Molecular Biology, Cancer Research Institute, Sapporo Medical University, Sapporo, Japan 4 PRESTO, JST, Kawaguchi, Japan 5 Department of Public Health, Sapporo Medical University, Sapporo, Japan 6 Department of Clinical Laboratory Science, Yamaguchi University School of Medicine, Ube, Japan

    Sequencing:

    Article Title: Pepper yellow leaf curl Aceh virus: a recombinant replacing the parental begomovirus is highly virulent and breaks Ty-1-mediated resistance in tomato
    Article Snippet: Begomovirus infections have threatened the global production of multiple crops, including Solanaceae species.. Recombination, a frequent phenomenon in begomoviruses, has profound effects on viral populations, allowing them to adapt to changing environments.. We previously isolated pepper yellow leaf curl Aceh virus (PepYLCAV) from pepper (Capsicum annuum), tomato (Solanum lycopersicum), and tobacco (Nicotiana tabacum) plants in Aceh Province, Indonesia, and determined that it is a recombinant of the widespread pepper yellow leaf curl Indonesia virus (PepYLCIV).

    Marker:

    Article Title: Pepper yellow leaf curl Aceh virus: a recombinant replacing the parental begomovirus is highly virulent and breaks Ty-1-mediated resistance in tomato
    Article Snippet: Begomovirus infections have threatened the global production of multiple crops, including Solanaceae species.. Recombination, a frequent phenomenon in begomoviruses, has profound effects on viral populations, allowing them to adapt to changing environments.. We previously isolated pepper yellow leaf curl Aceh virus (PepYLCAV) from pepper (Capsicum annuum), tomato (Solanum lycopersicum), and tobacco (Nicotiana tabacum) plants in Aceh Province, Indonesia, and determined that it is a recombinant of the widespread pepper yellow leaf curl Indonesia virus (PepYLCIV).

    Methylation:

    Article Title: Upregulation of BNIP3 by 5-aza-2'-deoxycytidine sensitizes pancreatic cancer cells to hypoxia-mediated cell death.
    Article Snippet: 1 First Department of Internal Medicine, Sapporo Medical University, South 1, West 16, Chuo-ku, Sapporo 060-8543, Japan 2 Department of Gastroenterology, Sapporo Kosei General Hospital, Sapporo, Japan 3 Department of Molecular Biology, Cancer Research Institute, Sapporo Medical University, Sapporo, Japan 4 PRESTO, JST, Kawaguchi, Japan 5 Department of Public Health, Sapporo Medical University, Sapporo, Japan 6 Department of Clinical Laboratory Science, Yamaguchi University School of Medicine, Ube, Japan

    Polymerase Chain Reaction:

    Article Title: PCR/restriction fragment length polymorphism (RFLP) typing of human and poultry Campylobacter jejuni strains.
    Article Snippet: GeneRulerTM 100 bp DNA Ladder Plus (MBI Fermentas) was used as a standard. .. PCR products (10 ll) were digested in 25 ll reaction buffer with 3 U of restriction enzyme AfaI, MboI and HaeIII (TaKaRa). ..



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    Fig. 2. Restriction fragment length polymorphism profiles of 16S rRNA gene amplified by nested polymerase chain reaction using prim er pair R16F2n/R16R2 with digested <t>AfaI</t> <t>(RsaI).</t> Lane M, molecular weight marker (100 bp); lanes 1-7, JJ011-JJ017; lanes 8-14, JS011- JS017; lane 15, Elaeocarpus sylvestris, decline phytoplasma ESDP-JJ1 (GenBank accession no. OL689203).
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    Fig. 2. Restriction fragment length polymorphism profiles of 16S rRNA gene amplified by nested polymerase chain reaction using prim er pair R16F2n/R16R2 with digested <t>AfaI</t> <t>(RsaI).</t> Lane M, molecular weight marker (100 bp); lanes 1-7, JJ011-JJ017; lanes 8-14, JS011- JS017; lane 15, Elaeocarpus sylvestris, decline phytoplasma ESDP-JJ1 (GenBank accession no. OL689203).
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    Fig. 2. Restriction fragment length polymorphism profiles of 16S rRNA gene amplified by nested polymerase chain reaction using prim er pair R16F2n/R16R2 with digested <t>AfaI</t> <t>(RsaI).</t> Lane M, molecular weight marker (100 bp); lanes 1-7, JJ011-JJ017; lanes 8-14, JS011- JS017; lane 15, Elaeocarpus sylvestris, decline phytoplasma ESDP-JJ1 (GenBank accession no. OL689203).
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    Fig. 2. Restriction fragment length polymorphism profiles of 16S rRNA gene amplified by nested polymerase chain reaction using prim er pair R16F2n/R16R2 with digested <t>AfaI</t> <t>(RsaI).</t> Lane M, molecular weight marker (100 bp); lanes 1-7, JJ011-JJ017; lanes 8-14, JS011- JS017; lane 15, Elaeocarpus sylvestris, decline phytoplasma ESDP-JJ1 (GenBank accession no. OL689203).
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    Fig. 2. Restriction fragment length polymorphism profiles of 16S rRNA gene amplified by nested polymerase chain reaction using prim er pair R16F2n/R16R2 with digested <t>AfaI</t> <t>(RsaI).</t> Lane M, molecular weight marker (100 bp); lanes 1-7, JJ011-JJ017; lanes 8-14, JS011- JS017; lane 15, Elaeocarpus sylvestris, decline phytoplasma ESDP-JJ1 (GenBank accession no. OL689203).
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    TaKaRa blunt end restriction enzymes afai
    Fig. 2. Restriction fragment length polymorphism profiles of 16S rRNA gene amplified by nested polymerase chain reaction using prim er pair R16F2n/R16R2 with digested <t>AfaI</t> <t>(RsaI).</t> Lane M, molecular weight marker (100 bp); lanes 1-7, JJ011-JJ017; lanes 8-14, JS011- JS017; lane 15, Elaeocarpus sylvestris, decline phytoplasma ESDP-JJ1 (GenBank accession no. OL689203).
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    TaKaRa restriction enzymes afai
    Fig. 2. Restriction fragment length polymorphism profiles of 16S rRNA gene amplified by nested polymerase chain reaction using prim er pair R16F2n/R16R2 with digested <t>AfaI</t> <t>(RsaI).</t> Lane M, molecular weight marker (100 bp); lanes 1-7, JJ011-JJ017; lanes 8-14, JS011- JS017; lane 15, Elaeocarpus sylvestris, decline phytoplasma ESDP-JJ1 (GenBank accession no. OL689203).
    Restriction Enzymes Afai, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/restriction+enzyme+afai/Afa+I/pm22048641-74-48-76
    Average 93 stars, based on 1 article reviews
    restriction enzymes afai - by Bioz Stars, 2026-09
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    Image Search Results


    Fig. 2. Restriction fragment length polymorphism profiles of 16S rRNA gene amplified by nested polymerase chain reaction using prim er pair R16F2n/R16R2 with digested AfaI (RsaI). Lane M, molecular weight marker (100 bp); lanes 1-7, JJ011-JJ017; lanes 8-14, JS011- JS017; lane 15, Elaeocarpus sylvestris, decline phytoplasma ESDP-JJ1 (GenBank accession no. OL689203).

    Journal: The Plant Pathology Journal

    Article Title: Molecular Detection of Phytoplasmas of the 16SrⅠ and 16SrXXXⅡ Groups in Elaeocarpus sylvestris Trees with Decline Disease in Jeju Island, South Korea

    doi: 10.5423/ppj.oa.07.2022.0091

    Figure Lengend Snippet: Fig. 2. Restriction fragment length polymorphism profiles of 16S rRNA gene amplified by nested polymerase chain reaction using prim er pair R16F2n/R16R2 with digested AfaI (RsaI). Lane M, molecular weight marker (100 bp); lanes 1-7, JJ011-JJ017; lanes 8-14, JS011- JS017; lane 15, Elaeocarpus sylvestris, decline phytoplasma ESDP-JJ1 (GenBank accession no. OL689203).

    Article Snippet: RFLP analysis was conducted with the restriction enzyme AfaI (RsaI) (TaKaRa, Otsu, Japan).

    Techniques: Amplification, Nested PCR, Molecular Weight, Marker